Brandon:LabNotes/Project1/2015-7-24

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NXTA_ME_B HiSeq, MiSeq troubleshooting, libraries that worked on HiSeq on MiSeq[edit]


  • gel size selection amounts (see 7/24/15 size selection column on far right)
gel size selection amounts ' ' ' ' '
4/2/2015 intensity ng/ul
7213.490215 5 for 7/24/2015 gel size selection
5410.760161 2.5 for 80 ng
1. idx49, t7-top2, 5X tn5-059, 500 cells rep1 19053.90057 21.42011006 3.734808075 7.46961615
2. idx50, t7-top2, 5X tn5-059, 500 cells rep2 16376.87049 17.70764341 4.517823076 9.035646151
3. idx51, t7-top2, 5X ez-tn5, 500 cells rep1 18371.57055 20.47386464 3.907420578 7.814841156
4. idx52, t7-top2, 5X ez-tn5, 500 cells rep2 16685.3305 18.13541129 4.411259205 8.82251841
5. idx53, t7-top2, 5X tn5-059, 6 ng pure DNA 38879.07116 48.91337028 1.635544628 0.8 uL 3.271089256
6. idx54, t7-top2, 5X ez-tn5, 6 ng pure DNA 34297.93102 42.56031131 1.879685499 0.8 uL 3.759370998
7. idx55, t7-top2, 5X tn5-059, NTC -2643.330079 -8.669296012 -9.227969594 1 uL -18.45593919
8. idx56, t7-top2, 5X ez-tn5, NTC -1150.120034 -6.59853389 -12.1239053 1 uL -24.2478106


  • gel size selection results

File:ZhangLab 2 2015-07-24 11hr 11min-labeled.jpg


  • gel size selection validation

File:ZhangLab 2 2015-07-24 14hr 04min-labeled.jpg

Results[edit]

  • samples did not work well on MiSeq, and did the same thing as other samples, with very low read numbers. Thus must be MiSeq problem.
    • loading?
    • special miseq reagents being used?