Dinh/NOTES/2010-7-3
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Sanger sequencing of PCR product with probe linker[edit]
Primers[edit]
LinkerR-LMNA-F: GTT GGA GGC TCA TCG TTC CTA TTT TGG GCC TGA GTG GTC AGT C LinkerL-LMNA-R: GAC CCG CCT GCA GGA TTT GGC AGG CAG ATG TTA TCG AGG TCC GAC Product length: 373+23bp+25bp= 421bp Map to position: chr1:156,108,195-156,108,590
PCR[edit]
Template (1-2ng/ul): 3ul F primer (10uM) : 1ul R primer (10uM) : 1ul H20 : 20ul Taq 2x MM : 25ul ----------------------- 50ul Templates used: HGPS-ips
Program: 94C 2min 94C 30s 62C 40s 72C 40s Go to 2 for 7x 94C 30s 72C 40s Go to 7 for 23x 72C 3min 15C forever
Results[edit]
PAGE[edit]
File:Attaching linker to LMNA amplicons.jpg