Jie:LabNotes/CpgSeq/2009-6-23
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I ordered 17 pairs of primers to validate the ASE of those fuzzy methylated genes.
I found the SNPs information in NCBI, then find those informative SNPs in Hapmap SNP database. Two families in which the ASE could be differentiated were selected:
Father Mother child 12872 12873 12864 12874 12875 12865
Media: 20090623_primers_fuzzylmethyl_ASE_valid.xls20090623_primers_fuzzylmethyl_ASE_valid