Jie:LabNotes/CpgSeq/2009-8-1

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ASM validation of SNP_ASM overlap high r2 region[edit]

SNP_ASM overlap high r2 region was given by Robert.
Primers to validate the SNP_ASM overlap high r2 region
I did the PCR using 2x iQ supermix on:
rs1061726_LOC283788_BJiPS11
rs1084602_FLJ22662_BJiPS12
rs1087789_BJiPS11
rs3746459_NECAB3_BJiPS11
rs4768587_BJiPS12
rs7266947_BC036544_BJ
rs7315028_ALG10B_BJiPS11
I got the amplicons except rs4768587_BJiPS12
File:20090801 ASM overlap r2 and SNPASM.jpg20090801_ASM_overlap r2 and SNPASM

I did the egel purification and TA cloning. I sent 32 clones for each amplicons to Agencourt.
Plate_rs1061726_rs1087789_rs1087789;
Plate_rs3746459_rs7266947_rs7315028.
The sanger results validated the ASM of: 
chr20:28225961_rs1061726_LOC283788_BJiPS11
chr12:14611633_rs1084602_FLJ22662_BJiPS12(need more clone?)
chr20:31718894_rs3746459_NECAB3_BJiPS11 (need more clone?)
The ASM was not shown in chr12:61312267_rs1087789_BJiPS11; and the sanger reads were messy for    
rs7266947_BC036544_BJ
rs7315028_ALG10B_BJiPS11