Jie:LabNotes/CpgSeq/2009-9-2
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ASM validation of imprinted genes[edit]
I did the bisulfite conversion of HUES42, HUES63 and hFib2[edit]
The concentration after bisulfite conversion using zymo kit: HUES42: 81.1ng/ul HUES63: 36.5ng/ul; hFib2: 16.7ng/ul
PCR with SLC26A10 and SNRPN primers[edit]
SLC26A10: HUES42 and hFib2; SNRPN: HUES63;
TA cloning of the PCR products from the above three samples[edit]
I send the plate SLC26A10_hFib2_HUES42_SNRPN_Hues63. A1-C8:SLC26A10_hFib2; C9-F4:SLC26A10_HUES42; F5-H12:SNRPN_Hues63.