Jie:LabNotes/ExonomeSeq/2009-5-29

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PCR with AmpF/R6.3Sol and iProof on templates from Alice[edit]

                                      x2
   Template                 1ul      
   2X iProof Mastermix     50ul      100ul
   AmpF6.3SoL (10uM)        4ul       8ul
   AmpR6.3SoL (10uM)        4ul       8ul   
   50X SYBG I             0.8ul      1.6ul
   H2O                   41.2ul     82.4ul
   

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 4 -> (98C 10S -> 72C 20S) x 9 -> 72C 3 min -> 15C hold.