Noi/NOTES/2012-2-29
Jump to navigation
Jump to search
Link to calendar:[[1]]
ASM analysis of WGBB12878[edit]
- Directory: /media/SeqStore2/120209_HiSeq/HL_WGBS/
s_7_1_sequence.txt.gz s_7_2_sequence.txt.gz s_8_1_sequence.txt.gz s_8_2_sequence.txt.gz
- Concatenate and uncompressed data (no trimming required)
nohup less /media/SeqStore2/120209_HiSeq/HL_WGBS/*.gz > 12878.fastq
- In Master Execution Script, PipelineExecute-NP.pl
my $root = "/home/expresso/Noi_ASM/WGBS_12878/"; my $readlength = 100;
- Note: After finishing concatenate sequencing data into 12878.fastq, I got only 23.9 Gb data. I repeated again, but got the same amount of data. The problem was resolved by splitting the read into different chromosomes based on the size of each one as described by Dinh's page: http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/Dinh_2012/NOTES/2012-3-5