Noi/NOTES/2012-8-22
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- [Link to calendar]
- Received 16 cell pellet (1x10^6 cells/tube) samples from Guanghui
No. | Sample description | Mark on the tube | Lab ID |
1 | WT-ESC | H9-WT-ES | GL-1 |
2 | LRRK2-GS-ESC | H9-LRRK-ES | GL-2 |
3 | WT-NSCs | H9-WT-NSC-P9 | GL-3 |
4 | LRRK2-GS-NSCs | H9-LRRK-NSC-P9 | GL-4 |
5 | 2nd-iPSCs-WT#5 (early passage) | H9-WT-NSC-iPSC#5 P18 | GL-5 |
6 | 2nd-iPSCs-WT#3 (early passage) | H9-WT-NSC-iPSC#3 P18 | GL-6 |
7 | 2nd-iPSCs-LK2-GS#2 (early passage) | H9-LRRK-NSC-iPSC#2 P18 | GL-7 |
8 | 2nd-iPSCs-LK2-GS#6 (early passage) | H9-LRRK-NSC-iPSC#6 P18 | GL-8 |
9 | 2nd-iPSCs-WT#5 (late passage) | W5-ips P34 | GL-9 |
10 | 2nd-iPSCs-WT#3 (late passage) | W2-ips P34 | GL-10 |
11 | 2nd-iPSCs-LK2-G2#2 (late passage) | L2-ips P34 | GL-11 |
12 | 2nd-iPSCs-LK2-GS#6 (late passage) | L6-ips P34 | GL-12 |
13 | Cardiomyocyes | combine two tube to get 1M cells | GL-13 |
14 | WT-NSCs | FA52-#6-NSC | GL-14 |
15 | FA-NSCs | FA123#2-NSC | GL-15 |
16 | Corrected FA-NSCs | FA-GC-16-NEO-NSC | GL-16 |
- Sample code GL1-GL13 for Project 1
- Sample code GL14-GL16 for Project 2
- Extract gDNA 16 of GL and 3 of AL
GL= Eluted with AE buffer, volume 55ul AL= Eluted with AE buffer, volume 200ul
- Sample Info will be added later
gDNA concetration by Nanodrop
Sample ID | ng/ul | A260 | 260/280 | Yields (ug) |
GL-1 | 295.8 | 5.916 | 2.04 | 16.3 |
GL-2 | 220.6 | 4.412 | 2.07 | 12.1 |
GL-3 | 149.7 | 2.994 | 2.01 | 8.2 |
GL-4 | 172.3 | 3.445 | 2.06 | 9.5 |
GL-5 | 285.2 | 5.704 | 2.05 | 15.7 |
GL-6 | 296.6 | 5.932 | 2.03 | 16.3 |
GL-7 | 320.0 | 6.400 | 2.03 | 17.6 |
GL-8 | 339.3 | 6.786 | 2.04 | 18.7 |
GL-9 | 467.5 | 9.349 | 2.02 | 25.7 |
GL-10 | 431.7 | 8.633 | 2.01 | 23.7 |
GL-11 | 349.1 | 6.981 | 2.03 | 19.2 |
GL-12 | 234.5 | 4.691 | 2.04 | 12.9 |
GL-13 | 72.2 | 1.445 | 1.89 | 4.0 |
GL-14 | 137.3 | 2.747 | 2.01 | 7.6 |
GL-15 | 207.5 | 4.150 | 2.03 | 11.4 |
GL-16 | 135.5 | 2.710 | 2.00 | 7.5 |
AL-3 | 256.8 | 5.136 | 2.08 | 51.4 |
AL-4 | 142.1 | 2.842 | 2.04 | 28.4 |
AL-5 | 140.1 | 2.802 | 2.00 | 28.0 |
Bisulfite conversion[edit]
Lab IDs | Conc. (ng/ul) | Volume for 1200ng | LabID ID | H2O | Total amount (ng) | CT conversion reagent (ul) | Total volume (ul) |
GL-1 | 295.8 | 4.06 | GL-1 | 15.94 | 1200.00 | 130.00 | 150.00 |
GL-2 | 220.6 | 5.44 | GL-2 | 14.56 | 1200.00 | 130.00 | 150.00 |
GL-3 | 149.7 | 8.02 | GL-3 | 11.98 | 1200.00 | 130.00 | 150.00 |
GL-4 | 172.3 | 6.97 | GL-4 | 13.03 | 1200.00 | 130.00 | 150.00 |
GL-5 | 285.2 | 4.21 | GL-5 | 15.79 | 1200.00 | 130.00 | 150.00 |
GL-6 | 296.6 | 4.05 | GL-6 | 15.95 | 1200.00 | 130.00 | 150.00 |
GL-7 | 320.0 | 3.75 | GL-7 | 16.25 | 1200.00 | 130.00 | 150.00 |
GL-8 | 339.3 | 3.54 | GL-8 | 16.46 | 1200.00 | 130.00 | 150.00 |
GL-9 | 467.5 | 2.57 | GL-9 | 17.43 | 1200.00 | 130.00 | 150.00 |
GL-10 | 431.7 | 2.78 | GL-10 | 17.22 | 1200.00 | 130.00 | 150.00 |
GL-11 | 349.1 | 3.44 | GL-11 | 16.56 | 1200.00 | 130.00 | 150.00 |
GL-12 | 234.5 | 5.12 | GL-12 | 14.88 | 1200.00 | 130.00 | 150.00 |
GL-13 | 72.2 | 16.61 | GL-13 | 3.39 | 1200.00 | 130.00 | 150.00 |
GL-14 | 137.3 | 8.74 | GL-14 | 11.26 | 1200.00 | 130.00 | 150.00 |
GL-15 | 207.5 | 5.78 | GL-15 | 14.22 | 1200.00 | 130.00 | 150.00 |
GL-16 | 135.5 | 8.86 | GL-16 | 11.14 | 1200.00 | 130.00 | 150.00 |
AL-1 | 226.6 | 5.30 | AL-1 | 14.70 | 1200.00 | 130.00 | 150.00 |
AL-2 | 375.5 | 3.20 | AL-2 | 16.80 | 1200.00 | 130.00 | 150.00 |
AL-3 | 256.8 | 4.67 | AL-3 | 15.33 | 1200.00 | 130.00 | 150.00 |
AL-4 | 142.1 | 8.45 | AL-4 | 11.55 | 1200.00 | 130.00 | 150.00 |
AL-5 | 140.1 | 8.56 | AL-5 | 11.44 | 1200.00 | 130.00 | 150.00 |
Program 1. 98°C for 8 minutes 2. 54°C for 60 minutes 3. 4°C hold
- Elute with 30ul Elution buffer, after Qubit quantification I have ~25ul for BSPP capture
- Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-8-23