Noi/NOTES/2013-3-20
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- [Link to calendar]
- Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2013-2-7
Skin Microbiom Project[edit]
- Sequencing libraries: NP-SkinMB_N2_Ind17-88_Feb07.2013
- Chris selected the top 20 libraries from analysis he did on light sequencing from the previous run (HL144, HL145) for Hiseq run.
- I pooled equimolar of these libraries (~100ng/library) and do PAGE size-selection (300-600bp) as I did in the previous pool.
Index | Well ID | Conc. (ng/ul) | Volume for 100ng (ul) |
24 | H3 | 8.37 | 11.95 |
34 | B5 | 29.56 | 3.38 |
36 | D5 | 30.58 | 3.27 |
37 | E5 | 29.43 | 3.40 |
44 | D6 | 13.98 | 7.15 |
48 | H6 | 4.43 | 22.57 |
54 | F7 | 16.68 | 6.00 |
55 | G7 | 13.04 | 7.67 |
56 | H7 | 9.28 | 10.78 |
58 | B8 | 9.28 | 10.78 |
59 | C8 | 10.88 | 9.19 |
66 | B9 | 7.70 | 12.99 |
73 | A10 | 4.78 | 20.92 |
74 | B10 | 10.83 | 9.23 |
76 | D10 | 13.33 | 7.50 |
78 | F10 | 10.81 | 9.25 |
80 | H10 | 6.92 | 14.45 |
84 | D11 | 12.25 | 8.16 |
86 | F11 | 11.91 | 8.40 |
88 | H11 | 9.89 | 10.11 |
- Library ID : CW-SkinMB-20SAG-Mar20. Run in HiSeq PE 100bp lane 4 (Alan did qubit quantification).
- Chris or Jeff will take care of data analysis