Rui:LabNotes/Collaborations/2011-7-8

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Quantification by gel[edit]

Covaris shearing DNAs[edit]

  1. Add 66ul purification buffer to ~8ul DNA samples
  2. Transfer 75ul mixture to Covaris tube
  3. Covaris location: Cancer center, 3rd, Rm.3352; Open Kun Zhang lab folder, use 200bp-5min.pro file; put sample in; start
Duty% Intensity Burst cycle Time (min) Mode
10 5 200 5min frequency sweeping
  1. Quick spin and transfer to PCR tubes if continue with library progress; if stop, store DNA in non-stick tube (1.5ml or 0.5ml)