Rui:LabNotes/Collaborations/2011-7-8
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Quantification by gel[edit]
Covaris shearing DNAs[edit]
- Add 66ul purification buffer to ~8ul DNA samples
- Transfer 75ul mixture to Covaris tube
- Covaris location: Cancer center, 3rd, Rm.3352; Open Kun Zhang lab folder, use 200bp-5min.pro file; put sample in; start
Duty%
|
Intensity
|
Burst cycle
|
Time (min)
|
Mode
|
10 |
5 |
200 |
5min |
frequency sweeping
|
- Quick spin and transfer to PCR tubes if continue with library progress; if stop, store DNA in non-stick tube (1.5ml or 0.5ml)