Rui:LabNotes/SingleCell/2011-6-4

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Kapa PCR for ER EL[edit]

  • EL sample is on 5.26.11
  • ER sample is on 6.2.11
  • 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13

QPCR curve[edit]

File:QPCR 6.4.11.jpg

TBE PAGE[edit]

File:TBE PAGE 6.4.11.jpg


Size selection for sequencing[edit]

  1. run TBE-PAGE, cut bands around 300bp-700bp
  2. finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer)
  3. Gel check for selected library was in lab note 6.6.11 [1]
  4. Mixed with haplotyping samples for sequencing, sample ID: RL-Hap_RNAseq-1_13-Jun6