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==Background== * Noi made a batch of WGBS libraries on the whole blood DNA from Dr. Ranaβs lab. * Dr. Rana has obtained some sequencing fund, and would like to get them sequenced. * Noi's experiment notes on wiki [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2014-2-3]] * Noi's wiki page shows that the library preparation failed and didn't have time to repeat experiment * Dinh found the gDNA samples from Dr. Rana in Noi's box. * From the labels, Noi got about 1 ug of the samples and only used 200 ng, we should have 800ng for library prep. * Noi confirmed that the experiment failed for the first round of experiment and she did try a second time and still the result was not very good * She got very faint smear or barely see a smear in many samples by amplifying about 16-18 cycles, which is very unlikely for DNA input ~100ng * Noi doesn't know why this is the case, may be it is the expired beads used for purification but I will give a a try. * I will repeat the library prep follow the exact protocol that you did with the tumor WGBS libraries. Using new beads and switch to Kapa Hyper for library preparation.
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