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=Combo-Lock= [[Daniel Jacobsen|Back to Main]] This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibody probes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universal padlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing. ==Assorted Lab Notes== [[Daniel:Notebook/ComboLock/IP|IP Presentations]] [[Daniel:Notebook/ComboLock/Polyacrylamide|PA Gel Formulas]] [[Daniel:Notebook/ComboLock/Devices|Microfluidic Devices]] [[Daniel:Notebook/ComboLock/CProbeDesign|C Probe Design]] [[Daniel:Notebook/ComboLock/ExperimentList|Experiment List]] [[Daniel:Notebook/ComboLock/Sequencing|Sequencing Lists]] ==Calendar== <calendar> name=Daniel:Notebook/ComboLock format=%name/%year-%month-%day date=2017/02/01 view=oneyear </calendar> <calendar> name=Daniel:Notebook/ComboLock format=%name/%year-%month-%day date=2016/02/01 view=oneyear </calendar>
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