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=Positive Control Amplicon Test 2 (Started [[Daniel:Notebook/ComboLock/2016-8-22|Monday]])= [[Daniel:Notebook/ComboLock|Back to Calendar]] ==Protocol-Library Prep== <ol start="7"> <li>Ethanol Precipitation (from [[Daniel:Notebook/ComboLock/2016-8-25|yesterday]])</li> <ol type="A" start="3"> <li>Centrifuge at 12000rpm at 4C for 25 minutes</li> <li>Remove supernatant and add 750 uL 70% EtOH</li> <li>Centrifuge at 12000rpm at 4C for 15 minutes</li> <li>Remove supernatant and let dry in PCR hood for 15 min</li> <li>Resuspend in 30 uL nfH2O</li> </ol></ol> ===Nanodrop Results=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="background-color:#C4BD97;font-size:12pt;font-weight:bold" align="center" | width="145" height="30" | Sample | width="65" | ng/uL ssDNA |- style="font-size:12pt" | height="15" valign="bottom" | 20160822 Sample A | align="center" align="center" valign="bottom" | 38.4 |- style="background-color:#D9D9D9;font-size:12pt" | height="15" valign="bottom" | 20160822 Sample B | align="center" align="center" valign="bottom" | 30.1 |}
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