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=Experiment List= [[Daniel:Notebook/ComboLock|Back to Calendar]] This page is a list of experiments performed in ComboLock and the corresponding dates to those experiments ==2017== ===July 2017=== <ol> <li>Protein/Oligo Mixture (7/6/2017)</li> <dynamicpagelist> category=ComboLock category=20170706 </dynamicpagelist> <li>Multiplex Experiment (7/13/2017)</li> <dynamicpagelist> category=ComboLock category=20170713 </dynamicpagelist> </ol> ===June 2017=== <ol> <li>Sensitivity and Specificity Experiments (6/5/2017)</li> <dynamicpagelist> category=ComboLock category=20170605 </dynamicpagelist> <li>Antibody Testing (6/14/2017)</li> <dynamicpagelist> category=ComboLock category=20170614 </dynamicpagelist> </ol> ===May 2017=== <ol> <li>BSA Antibody production (5/13/2017)</li> <dynamicpagelist> category=ComboLock category=20170513 </dynamicpagelist> </ol> ===April 2017=== <ol> <li>RCA Test-2 primers "Double Deuce or DD" (4/11/2017)</li> <dynamicpagelist> category=ComboLock category=20170411 </dynamicpagelist> <li>Production Run-Test final parameters and such; full protocol (4/28/2017)</li> <dynamicpagelist> category=ComboLock category=20170428 </dynamicpagelist> </ol> ===March 2017=== <ol> <li>Version 3 Oligos-Padlock0601 Test (3/1/2017)</li> <dynamicpagelist> category=ComboLock category=20170301 </dynamicpagelist> <li>Bolt Test-Ligation reaction connecting the C probes for a padlock binding target (3/8/2017)</li> <dynamicpagelist> category=ComboLock category=20170308 </dynamicpagelist> <li>T4Poly-Testing Circularization methods including t4 polymerase/ligase and hot-starting phusion (3/15/2017)</li> <dynamicpagelist> category=ComboLock category=20170315 </dynamicpagelist> <li>Creating a Circular/Linear Product for Positive/Negative RCA Controls (3/22/2017)</li> <dynamicpagelist> category=ComboLock category=20170322 </dynamicpagelist> <li>RCA Test on Circular and Linear Controls (3/26/2017)</li> <dynamicpagelist> category=ComboLock category=20170324 </dynamicpagelist> </ol> ===February 2017=== <ol> <li>Version 2 Oligos Test 2 (2/2/2017)</li> <dynamicpagelist> category=ComboLock category=20170202 </dynamicpagelist> <li>Version 3 Oligos-Low Temps (2/7/2017)</li> <dynamicpagelist> category=ComboLock category=20170207 </dynamicpagelist> <li>Beads Binding Test (2/9/2017)-Tests the efficiency of binding for the biotin-template to the beads and especially the C probes to the template. Also focuses on the temperature-dependent efficiency of wash/incubation steps</li> <dynamicpagelist> category=ComboLock category=20170209 </dynamicpagelist> </ol> ===January 2017=== <ol> <li>Version 2 Oligos - Short Oligonucleotides (1/9/2017)</li> <dynamicpagelist> category=ComboLock category=20170106 </dynamicpagelist> <li>Version 3 Oligos - Normal Length Adapters, Lock Oligos, Phosphates Attached (1/13/2017)</li> <dynamicpagelist> category=ComboLock category=20170113 </dynamicpagelist> <li>RCA Test Dilutions - (1/23/2017) - Uses C4-C2 amplicon and Padlock0501 to test circularization and RCA effects</li> <dynamicpagelist> category=ComboLock category=20170123 </dynamicpagelist> <li>RCA Time Test - (1/27/2017) - Testing Various RCA conditions incl time</li> <dynamicpagelist> category=ComboLock category=20170127 </dynamicpagelist> </ol> ==2016== ===December 2016=== <ol> <li>Mirror C Probe - Overnight capture (12/15/2016)</li> <dynamicpagelist> category=ComboLock category=20161215 </dynamicpagelist> <li>Mirror C Probe Test (12/13/2016)</li> <dynamicpagelist> category=ComboLock category=20161213 </dynamicpagelist> </ol> ===November 2016=== <ol> <li>Antibody Binding Positive Control Test (11/03/2016)</li> <dynamicpagelist> category=ComboLock category=20161103 </dynamicpagelist> <li>Cell Test with RCA (11/07/2016)</li> <dynamicpagelist> category=ComboLock category=20161107 </dynamicpagelist> <li>Antibody Binding Positive Control Test 2 (11/15/2016)</li> <dynamicpagelist> category=ComboLock category=20161115 </dynamicpagelist> <li>Circularization Test 2 (11/22/2016)</li> <dynamicpagelist> category=ComboLock category=20161122 </dynamicpagelist> <li>Extended Latch Test (11/29/2016)</li> <dynamicpagelist> category=ComboLock category=20161129 </dynamicpagelist> </ol> ===October 2016=== <ol> <li>C-Probe Positive Control Test (10/05/2016)</li> <dynamicpagelist> category=ComboLock category=20161005 </dynamicpagelist> <li>Padlock0301 Test (C-Probe Positive Control Template; 10/07/2016)</li> <dynamicpagelist> category=ComboLock category=20161007 </dynamicpagelist> <li>Padlock0301 Dilutions Test (10/13/2016)</li> <dynamicpagelist> category=ComboLock category=20161013 </dynamicpagelist> <li>BSA Ab-Oligo Linkage (10/14/2016)</li> <dynamicpagelist> category=ComboLock category=20161014 </dynamicpagelist> <li>Cell Test with Padlock0201 (10/17/2016)</li> <dynamicpagelist> category=ComboLock category=20161017 </dynamicpagelist> <li>Cell Test 2 (10/18/2016)</li> <dynamicpagelist> category=ComboLock category=20161018 </dynamicpagelist> <li>Cell Test 3-Time Course Experiment (10/21/2016)</li> <dynamicpagelist> category=ComboLock category=20161021 </dynamicpagelist> <li>Beads UMI Test (10/27/2016)</li> <dynamicpagelist> category=ComboLock category=20161027 </dynamicpagelist> <li>Circularization Test (10/31/2016)</li> <dynamicpagelist> category=ComboLock category=20161031 </dynamicpagelist> </ol> ===September 2016=== <ol> <li>Positive Control Amplicon PCR Test, Primer Concentration (09/22/2016)</li> <dynamicpagelist> category=ComboLock category=20160922 </dynamicpagelist> <li>PCAmp4 Production (09/28/2016)</li> <dynamicpagelist> category=ComboLock category=20160928 </dynamicpagelist> <li>PC amplification test using (P12RC/P2) amplification primers(09/29/2016)</li> <dynamicpagelist> category=ComboLock category=20160929 </dynamicpagelist> <li>Padlock0201 test including primer amplification (Adapter is now primer24 sequence; 09/30/2016)</li> <dynamicpagelist> category=ComboLock category=20160930 </dynamicpagelist> </ol> ===August 2016=== <ol> <li>Positive Control Amplicon Production (08/05/2016)</li> <dynamicpagelist> category=ComboLock category=20160805 </dynamicpagelist> <li>Positive Control Amplicon Test (08/18/2016)</li> <dynamicpagelist> category=ComboLock category=20160818 </dynamicpagelist> <li>Positive Control Amplicon Production 2 (08/23/2016)</li> <dynamicpagelist> category=ComboLock category=20160823 </dynamicpagelist> <li>Positive Control Amplicon Test 2 (08/22/2016)</li> <dynamicpagelist> category=ComboLock category=20160822 </dynamicpagelist> <li>Positive Control Dilution-X2(08/26/2016)</li> <dynamicpagelist> category=ComboLock category=20160826 </dynamicpagelist> <li>Positive Control-X3 (08/29/2016)</li> <dynamicpagelist> category=ComboLock category=20160829 </dynamicpagelist> </ol> ===July 2016=== <ol> <li>C Probe SOD1/VIM Test 2 (7-1-2016)</li> <dynamicpagelist> category=ComboLock category=20160701 </dynamicpagelist> <li>Sequential Hybridization Test (7-5-2016)</li> <dynamicpagelist> category=ComboLock category=20160705 </dynamicpagelist> <li>Double Hybridization (C Probes+Antibodies)</li> <dynamicpagelist> category=ComboLock category=20160707 </dynamicpagelist> <li>Million Fold Test (SOD1)</li> <dynamicpagelist> category=ComboLock category=20160711 </dynamicpagelist> <li>C Probes (VIM+SOD1) with Stringent Washes</li> <dynamicpagelist> category=ComboLock category=20160718 </dynamicpagelist> <li>Latch 100X and Dextran Test</li> <dynamicpagelist> category=ComboLock category=20160725 </dynamicpagelist> <li>Phusion Test</li> <dynamicpagelist> category=ComboLock category=20160728 </dynamicpagelist> </ol> ===June 2016=== <ol> <li>Stage 2 Test (C Probe, VIM, 6-23-2016)</li> <dynamicpagelist> category=ComboLock category=20160623 </dynamicpagelist> <li>Antibody Stage 2 Test 1 (6-27-2016)</li> <dynamicpagelist> category=ComboLock category=20160627 </dynamicpagelist> <li>Antibody Stage 2 Test 2 (6-28-2016)</li> <dynamicpagelist> category=ComboLock category=20160628 </dynamicpagelist> <li>C Probe SOD1/VIM Test 1 (6-29-2016)</li> <dynamicpagelist> category=ComboLock category=20160629 </dynamicpagelist> </ol>
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