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=Probe Mining-Stellaris= [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] Designing another set of probes for dystrophin. Some of the other probe designing software takes a little while, but [www.biosearchtech.com|Stellaris] can generate a series of 20mer probes real quick. So I'm using that to design a few probes for the dystrophin gene that can be detected quickly. I'll be using a sparing amount for now, ordering 32 probes with an Amino modifier on the 5' end. This will allow the probes to utilize the ARES technology, and I should be able to generate a FISH experiment with only dye labeling step followed by purification and FISH (no DpnII or Lambda exonuclease). ===Workflow=== #Obtained CDS region of dystrophin gene using UCSC genome browser #Took first 8000bp (Stellaris maximum) and generated a 32bp probe set ##[[Media:Dystrophin_probes.txtβ|Probe list]] ##columns: probe# sequence startpos GCcontent [[Category:HRCP]] [[Category:Probe Mining]] [[Category:Stellaris]]
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