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== Use CoRE Protocol for MDA in Device v7 (MEF #2)== * Single MEF cell was planned and used in this protocol. The difference in mastermix preparation to the experiment on May17 was the additional dUTP in this CoRE protocol. * MDA was performed in the device for 15 hours at 30C, plus 1.5 hours at 20C. * Since I believe some MEF cells lost the DNA after a few hours in PBS on ice, all experiments will only use freshly trypsinized cells in culture media.
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