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== MDA Samples Collection from MDA Device v7 (MEF #2)== * Continued from yesterday # Samples were collected by TE buffer after 1.5 hour at 20C. TE buffer: 10mM Tris, 1mM EDTA, 0.2% Tween20 * note: a few valve lines were emptied over the course of 15 hours. Air was pushed into the fluidic layer. # TE buffer was loaded from the top. And the samples were all pushed lower and out of the chambers to avoid mixing. The center 8 chambers were collected first. And the samples of some chambers were back flowed. * Bubbles were generated from empty valve lines during sample collection. Video was taken when bubbles pushed the samples away from chamber 23, and that caused mixing. * note: back flowed can be avoided in the future by designing 2 sets of valves near the collection outlets. # Samples were collected by pipet tips and transferred to PCR tubes. # All chambers were collected in about 6ul volume. # 65C for 15min to deactivate Phi29
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