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Hosuk:Cell fixation in culture dish
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===Jonathan's protocol=== *100% confluence, and could wait one more day, and split *When cells are transferred from 6 well plate **500uL of Trypsin, incubate 5min **Add 500uL media, and mix well, and **100uL media in 96 well, or 300~350uL media in glass bottom dish **Add 60uL of cell in 96 well or 200uL in glass bottom dish **Culture one or two more day *Fixed cells just using 10% Formamide **Aspirate media **Add 50uL of 10% Formamide (200uL for glass bottom) **Incubate for 15min **Wash with PBS three times **Store in Ethanol
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