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===Split cells in 96 well plate=== =====Surface area of each culture dish===== *T25 flask = 25 cm2 *MatTek dish = 1.54 cm2 (1.4cm diameter) *6 well plate = 9.5 cm2 --> '''6 well : T25 flask = 1 : 2.6''' *96 well plate = 0.32 cm2 --> '''96 well : MatTek dish = 1 : 4.8''' ====Jonathan’s cell split ==== *He has cultured cells in 6 well plate. *Add 500uL Trypsin, incubate *Add 500uL media (total 1mL) *Add 100uL media to each well in 96 well plate *Add 60uL cell (from 1mL in 6 well plate) to each well in 96 well plate ====What I did today is…==== *Add 1mL Trypsin in T25 flask *Add 4mL media (total 5mL) *Add 100uL cell (from 5mL in T25 flask) to each well in 96 well plate (total 16 wells) *Add 200uL media to each well, and gently mix
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