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===Reverse Transcription check=== *based on Debugging plan ([[Hosuk:LabNotes/2013-5-1|05/01]]) =====Result===== *RT process seemed work. *+RTase sample showed very strong Cy5 signal which is supposed cDNA *comparing to -RTase result, the bright singal over the cell seemed they were cDNAs *-RTase sample has very low intensity of signals in the cell, and *not many spots were observed, which spots were outside of cells. *Most of signal came from cell structure. *The picture of -RTase is the position where the most number of spots were observed in all over the area. *In the +RTase sample, similar number or density of spots were observed in all over the area. *I don't know what are the large spots. Are they also cDNAs? *'''+RTase''' {| {{table}} | S2:+RTase, Brighfield, 20x || | S2:+RTase, Cy5, 20x || | S2:+RTase, Merged || |- |([[Media:RTCheck_+RTase_fig08_20xobj_pos04_BF_exp0.2-EM00.tif|Original]]) || |([[Media:RTCheck_+RTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.tif|Original]]) || | |- |[[File:RTCheck_+RTase_fig08_20xobj_pos04_BF_exp0.2-EM00.jpg|300px]] || |[[File:RTCheck_+RTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.jpg|300px]] || |[[File:Composite_+RTase_fig07-08.jpg|300px]] |} *'''-RTase (No RTase)''' {| {{table}} | S3:-RTase, Brighfield, 20x || | S3:-RTase, Cy5, 20x || | S3:-RTase, Merged || |- |([[Media:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.tif|Original]]) || |([[Media:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.tif|Original]]) || | |- |[[File:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.jpg|300px]] || |[[File:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.jpg|300px]] || |[[File:Composite_NoRTase_fig07-08.jpg|300px]] |} ---- ----
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