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== '''Test to see if DNA attaches to bead: SYBR Green Staining''' == ''Before we try and hybridize, I want to ensure that amplified DNA is attached to the bead. '' *Use product from MDA on 8-26-09 *Place PCR tube next to magnet, let beads assemble, and remove agarose gel *Add 20 uL of PBS and mix with pipette *Add 1 uL of 50X SYBR Green and mix *Incubate at RT in dark for 5 min *Pipette 5 uL onto a slide to view on a microscope '''Results''' The following were taken with a green filter. Product was observed, showing that the DNA was attached to the beads. [[Image:8-27-09 1.jpeg|400 px]] 20X mag [[Image:8-27-09 2.jpeg|400 px]] 10X mag [[Image:8-27-09 3.jpeg|400 px]] 50X mag
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