Editing
Jie:LabNotes/ASE/2008-9-22
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==padlock capture of sample from Yu Gao with eSNP84K== capture with the padlock probe made on 08-03-27. tube 1: G2 (~0.25ug/ul) tube 2: 2B (~3.5ng/ul) tube 3: 3B (~3.5ng/ul) tube 4: H1 (~5ng/ul) tube 5: H2 (~100ng/ul) tube 6: Jurkat DNA (5ng/ul) tube 7: negative control reaction system tube 1 x7 H2O 6.3ul 44.1ul 10x Ampligase Buffer 1ul 7ul probe(2008-03-27,30ng/ul) 3ul 21ul template 2ul 2ul each ddH2O Ligase Buffer Ligase Amplitaq dNTP 2U/ul AmpliTaq Stoffel fragment; 0.5U/ul AmpLigase; 0.8nM dNTP 0.2ul 0.1ul 0.1ul 0.2ul (2nM)0.4ul x20 4ul 2ul 2ul 4ul 8ul Reaction program: 95c 10min -> 63C 14h -> add 1ul SLN mix(2U/ul AmpliTaq Stoffel fragment; 0.5U/ul AmpLigase; 0.8nM dNTP) -> 60C 4h -> <BR>12 cycels (95C 1min -> 0.1C/sec to 60C, 60C for 4h 55C 1h) -> 95C 1min -> 37C 1min -> <BR>add 2ul Exonuclease I/III mix -> 37C 2h -> 94C 5min -> 4C hold.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information