Editing
Kun:LabNotes/ASE/2007-10-18
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
===Exp. 1 Perform PCR on CES22k and eSNP84k libraries to make MIP probes.=== Reaction CES22k(100nM): x 8 Template 0.2ul 1.6ul 10X buffer 10ul 80ul 10mM dNTP 2ul 16ul 100uM pAP1V6U 0.4ul 3.2ul 100uM phosAp2V6 0.4ul 3.2ul JumpStart Taq 2ul 16ul H2O 85ul 680ul Reactions eSNP84k set1-5 (100nM): x 8 x 5 Template 0.2ul 1.6ul x 5 10X buffer 10ul 80ul x 5 10mM dNTP 2ul 16ul x 5 100uM AP1V41U 0.4ul 3.2ul x 5 100uM Ap2V4 0.4ul 3.2ul x 5 JumpStart Taq 2ul 16ul x 5 H2O 85ul 680ul x 5 94C3min -> 20 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 4C hold [[image:2007-10-19-AgilentLib-PCR.png]] Estimated yield: CES22k: 12.5ng/ul eSNP84k1: 11.5ng/ul eSNP84k2: 13ng/ul eSNP84k3: 8.5ng/ul eSNP84k4: 12.75ng/ul eSNP84k5: 15.25ng/ul Ethanol Precipitation, 400ul reactions per tube, two tubes per sample. Resuspended 50ul dH2O, measured concentration with Nanodrop. NaOAC, pH=5 NaOAC, pH=7 CES22k: 215ng/ul 246ng/ul eSNP84k1: 216ng/ul 184ng/ul eSNP84k2: 184ng/ul 289ng/ul eSNP84k3: 157ng/ul 290ng/ul eSNP84k4: 142ng/ul 256ng/ul eSNP84k5: 163ng/ul 221ng/ul
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information