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===Exp1: Probe prep (cont.)=== *Starting from ethanol precipitated DNAs made at [[Kun:LabNotes/ASE/2007-10-18 |10/18/2007]].<br> *Pool two tubes of the same amplicons into ~100ul in total, add 11ul 10x Lambda Exo buffer, 4ul 5U/ul Lambda exonuclease. Incubate @ 37C for 2 hours, check 2ul of the products in both 8% TBE gels and 6% TBU gels. 8% TBE gel 6% TBU gel [[image:2007-10-29-PCR-Lambda_exo-TBE.jpg]] [[image:2007-10-29-PCR-Lambda_exo-TBU.jpg]] *I saw sharp bands in the non-denaturing gel, suggesting there are still a lot of dsDNA. I decided to incubate the reactions for another 4 hours. I probably used the wrong reverse primer for the eSNP84k sets. I should have used the phosAP2V4 instead of AP2V4, since lambda exonuclease doesn't cut without a 5'-phosphate group.
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