Editing
Kun:LabNotes/ASE/2008-1-6
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Exp.1 Real-time PCR on the capturing reactions from 01/02/08 and 01/03/08's reactions== x 20 x 2 Template: 10ul or 15ul 10X buffer: 9ul or 14ul 25mM dNTP: 0.8ul or 1.2ul 100uM AmpV6.2SolF 0.4ul or 0.6ul 100uM AmpV6.2SolR 0.4ul or 0.6ul 50X SYBGI: 0.4ul or 0.6ul JumpStart Taq 2ul or 3ul H2O: 77ul or 115ul 94C 3min -> (94C 45 sec -> 57C 1min -> 72C 1min) x 10 -> (94C 30min -> 72C 1min) x 8 -> 72C 3min -> 15C hold. The amplification curves didn't come up at the end of PCR. Then I realized that I should be using the AmpSolF2/AmpSolR primer pair. I added 0.5ul of 100uM primers to each reaction, repeat the PCR. The amplification curves came up, but I saw many non-specific bands in the PAGE gel. [[Image:2008-01-07-eSNP84k-capture-PCR.png|500px]]
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information