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===Expected number of ACTB and RAB7A Rolonies=== *Initial trial of secondary gene rolony generation showed low number of rolonies http://genome-tech.ucsd.edu/LabNotes/index.php/Hosuk:LabNotes/2013-9-12#Number_of_Rolonies *I want to look at literature to see how many rolonies of ACTB and RAB7A we should expect *http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM313643 is gene expression data for PGP1F cells using Illumina Ref-8 v3.0 beadchip (targets 18,631 unique genes) *The table at the end of http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL6883 can be used to convert gene symbol to ILMN ID Ref {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''ID Ref''' | align="center" style="background:#f0f0f0;"|'''ID Ref''' | align="center" style="background:#f0f0f0;"|'''Expression Value''' | align="center" style="background:#f0f0f0;"|'''Expression Value''' | align="center" style="background:#f0f0f0;"|'''% of total''' |- | ACTB||ILMN_2152131|| ILMN_2038777||42,723.45||33,579.69||0.32 |- | RAB7A||ILMN_1716524||N/A||7,254.71||N/A||0.030 |- | All Transcripts||N/A||N/A||23,976,360||N/A||100 |} *According to this: http://bfg.oxfordjournals.org/content/8/6/437.full.pdf, 95% of RNA is housekeeping ncRNA. In other words ~5% of our primary rolonies is mRNA *Therefore expect 0.016% of primary rolonies to be ACTB secondary rolonies *Expect 0.0015% of primary rolonies to be RAB7A secondary rolonies
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