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===Testing non-specific binding of Shifted FISSEQ=== ====Procedure==== Using negative controls (E7 & F7) from [[Matt:LabNotes/2014-1-15]] #Strip with 80% formamide for 10min @50C ##Rinse twice with H2O #Image 488 channel #Add 50ul 2uM Shifted FISSEQ preheated to 60C (1ul 200uM Dye + 30ul 100% formamide + 10ul 20X SSC + 59ul H2O) ##Incubate RT for 15min ##Wash 2 times with 2X SSC #Image 488 channel ====Results==== *More signals from non-specific binding than from actual experiment before **Since this is a negative control well it actually is a good sign in a way... *Continued checking for non-specific binding here: [[Matt:LabNotes/2014-1-27#Testing_non-specific_binding_of_Shifted_FISSEQ]]
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