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= Test BSPP DNA capture with DMR330k probe set with all new reagents = * '''Sample:''' * 3 human iPS lines with single Nonsynonymous mutations and 2 GA samples from Kang Zhang's lab ** 2HFF 8d-10 (conc. 214.6 ng/ul) ** 218HFF 2-11 (conc. 411.1 ng/ul) ** 218 HFF 4-11 (conc. 359.6 ng/ul) ** GA sample well A5 (conc. of bis-cvt DNA 88 ng/ul) ** GA sample well ID B5 (conc. of bis-cvt DNA 50.4 ng/ul) Note: Since S5 probe was lately made and I a little bit concern about the stuffs in the probe like salts or acrylamide contaminations even it never show the effect before (The volume of S5 probe is the largest in the reaction). I will set up the reaction with or without S5 probe. {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Sample ID in Zhang lab''' | align="center" style="background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''volume for 1200ng (ul)''' | align="center" style="background:#f0f0f0;"|'''H20 (ul)''' | align="center" style="background:#f0f0f0;"|'''Total amount for bis-cvt (ng)''' | align="center" style="background:#f0f0f0;"|'''CT conversion reagent (ul)''' | align="center" style="background:#f0f0f0;"|'''Total volume (ul)''' |- | 2HFF 8d_10||8d_10||214.60||5.59||14.41||1200.00||130.00||150.00 |- | 218HHF211||2_11||411.10||2.92||17.08||1200.00||130.00||150.00 |- | 218HHF411||4_11||359.60||3.34||16.66||1200.00||130.00||150.00 |} * Note: prepared two reactions for each samples<br> == Qubit ss-DNA assay == {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Conc. In Qubit (ng/mL)''' | align="center" style="background:#f0f0f0;"|'''Volume (ul)''' | align="center" style="background:#f0f0f0;"|'''Final conc (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Yields (ng)''' | align="center" style="background:#f0f0f0;"|'''Volume for 250ng''' |- | 8d_10||95.60||38.00||19.12||726.56||13.08 |- | 2_11||69.80||38.00||13.96||530.48||17.91 |- | 4_11||79.30||38.00||15.86||602.68||15.76 |}<br>
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