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= BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMAPPT01, nine replicates, HFF somatic lines, repeat UPenn samples, and comparing DNA methylation kit from Zymoresearch and Sigma = * '''Total number of reactions = 39 reactions''' ** '''9 samples from Sergio''' *** 1. H9 P43 mTeSR *** 2. H9 p43+Activin 100ng/mL 5days *** 3. H9 p43+BMP4 50ng/mL 5days *** 4. HUViPS4F1 p19 *** 5. HUViPS4F1 p19+Activin 100ng/mL 5days *** 6. HUViPS4F1 p19+BMP4 50ng/mL 5days *** 7. HUViPS4F3 *** 8. HUViPS4F3+Activin 100ng/mL 5days *** 9. HUViPS4F3+BMP4 50ng/mL 5days ** '''12 samples from 2 largest pedigrees of HAPMAPPT01''' *** Note: **** wellID = wellID from original HAPMAPPT01 plate **** All GM IDs (cell products) should be changed to NA (DNA products) *** Family 1362 **** GM11995 (wellID G7) **** GM11993 (wellID F2) **** GM10861 (wellID A9) **** GM11992 (wellID C2 ) **** GM11994 (wellID G9 ) **** GM10860 (wellID F10) *** Family 1454 **** GM12813 (wellID E8) **** GM12802 (wellID E7) **** GM12815 (wellID G12) **** GM12812 (wellID F11) **** GM12814 (wellID A7) **** GM12801 (wellID D9) ** '''10 replicates''' *** NA12156 (HAPMAPPT01, wellID B3) ** '''2 somatic lines''' *** 2HFF P6 *** 218 HFF P7 ** '''4 samples from Penn''' (repeat the low QC value samples from the first batch) ** '''2 reactions to compare bis-cvt gDNA quality processed by two different DNA methylation kits, Zymoresearch (DNA methylation gold kit) and Sigma (Imprint DNA modification kit)''' *** PGP1iPS * '''Note: For the gDNA of HAPMAPPT01, aliquot 10 ug to each of two working plates and save the original plate'''
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