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== Plan for probe preparation for Matt's project == * Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2013-2-27#Plan_for_probe_preparation_for_Matt.27s_project * 2013/03/06: received seed oligos from CustomArray * Seed oligo information ** 200 nt ** Total amount 4.95ug (61.93ng/ul in total volume 80ul in TE buffer) * Calculate MW of 200 nt probe = 200nt * 308.9D/bp + 79D = 61859 g/mol * 61.93ng/ul = 1.00114 ~ 1 uM or 1000 nM --> calculated from: 61.93(ng/ul)/61,859(g/mol) need to convert the unit to mol/L at the end * Aliquot 2ul of 61.93ng/ul oligos for PAGE verification & save original tube with Matt * Since the concentration is quit high, I will dilute to 100nM first (in 100ul) then dilute to 20uM (in 200ul) - To dilute from 1uM to 100nM volume 100ul: mix 10ul of 1uM oligos (61.93 ng/ul) with 90ul H2O - To dilute from 100nM to 20nM volume 200ul: mix 40ul of 100nM oligos with 160ul H2O === 3/07/2013 === ** After I diluted oligos to 100nM, I measured the oligo concentration by Nanodrop: 10.5 ng/ul (after dilute 10X, expected conc. ~ 6.1ng/ul)
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