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==Samples== ===Dr. Juan Carlos Izpisúa Belmonte lab=== * Alice Belmonte lab box (-80) * A.P. box (-80) * A.P. for Rui/Kun Zhang box (-80) May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C March 16th 2012: all colony/clone "B", Athanasia box P2 a single marked colony P3 a single marked colony P15 a single marked colony (X3) P30 half of a dense 6-well plate (X2) ====Table for samples from A.P (Belmonte lab)==== {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''hES cells''' | align="center" style="background:#f0f0f0;"|'''dFb''' | align="center" style="background:#f0f0f0;"|'''dFb GFP colonies''' | align="center" style="background:#f0f0f0;"|'''P0 Notes''' | align="center" style="background:#f0f0f0;"|'''P1''' | align="center" style="background:#f0f0f0;"|'''P2''' | align="center" style="background:#f0f0f0;"|'''P3''' | align="center" style="background:#f0f0f0;"|'''P5''' | align="center" style="background:#f0f0f0;"|'''P15''' | align="center" style="background:#f0f0f0;"|'''P30''' |- | gDNA||gDNA||A (A1, A2, A3)||A1, A2 protocol test||||single colony||single colony||||single colony|| |- | 100c (X3)||100c (X3)||B (B1, B2, B3)||B2, B3 -> DNAseq||||single colony||single colony||||single colony (X3)||gDNA |- | 1000c (X4)||1000c (X4)||C (C1, C2, C3)||||||single colony||single colony|||||| |- | ||||D (D2, D3, D4)||D1 is missing||single colony||single colony||||single well||single colony (X1) single well (X1)|| |- | ||||E |||||||||||||| |- | ||||F (F1, F2, F3)||F1 is missing||single colony||single colony||||single colony (X1) single well (X1)||single colony (X1) single well (X1)|| |- | ||||G||G is missing|||||||||||| |- | |||||||||||||||||| |- | ||||HUVEC colony||P0 notes||P1||P2||P3||P5||P10||P15 |- | ||HUVEC||D (D1, D2)||||||single colony||||single colony||single colony (X2) single well (X2)||single colony (X2) single well (X2) |} ===Dr. George Church lab=== * PGP1 BAC library: -80C, Rui BAC (PGP1) box ===Bulk DNA isolation=== * Promega wizard genomic DNA isolation kit
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