Editing
Tina:MESC rainbow line
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==mESC rainbow line expansion== *'''5/2''' **Received one vial of cells from Inna, and stored it in the N2(l) tank located at CERC. *'''5/3''' **discussed with Dr. Blue Lake in Dr. Yang Xu’s lab *'''5/7 preparing MEF with Blue''' *'''5/8 thawing''' **Thaw cells in one 6-well plate. Add 3 ml of [[mouse ES cell culture medium]] per well. *'''5/11''' **some colonies appear to show *'''5/14 Passaging''' **The culture looks ok, but there is some differentiation… so Blue passaged at several different dilutions (the lower ones to try and get rid of the differentiated cells). **Passage one 6 well into one 1:3, one 1:4, one 1:5, and one 1:6 well. *'''5/18 Passaging''' **Passage the 1:3 well into two 1:3 wells, two 1:6 wells, and one 1:12 well. **Frozen down 3 vials of P4 InsACGGsAlov3 in [[mouse ES cell freezing medium]] (from one 1:4 well, one 1:5 well, and one 1:6 well). *'''5/21 Passaging (Blue and Tina)''' **1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day. **Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) **Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) **Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well) *'''5/22''' **P6 cells look nice. Cells were passaged well yesterday. **P5 1:12 well shows a few differentiated colonies, and therefore is discarded to maintain good cell quality. *'''5/24 Passaging''' **Bring mESCs back to CERC. Will start to culture by myself. **Bring back 8 vials of P5 InsACGGsAlov3 and store them in the N2(l) tank located at CERC. **Passage one well of 1:6 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) **Frozen down 7 vials of P6 InsACGGsAlov3 (from two 1:3 well and one 1:6 well) *'''5/25''' **Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) **Frozen down 3 vials of P6 InsACGGsAlov3. Store at N2(l) tank, Box3, Location 16,17,18. *'''5/28''' **Passage: P7 to P8. Seed in 1:6 (1 well) and 1:12 (1 well). *'''5/31''' **Frozen down 3 vials of P8 InsACGGsAlov3. Store at N2(l) tank, Box3, Location 19,20,21. *'''6/1''' **Passage: P8 to P9. Seed in 1:6 (1 well, with MEF) and 1:3 (one well, without MEF feeder cells). Seed cells without MEF to test the proliferation ability of InsACGGsAlov3 cells under feeder free culture system. *'''6/4''' **Passage feeder free mESCs: P9 to P10. Seed in 1:6 (one well, without MEF). **Passage: P9 to P10. Seed in 1:6 (one well, with MEF). *'''6/6''' **Passage feeder free mESCs: P10 to P11. Seed in 1:12 (one well, without MEF).
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information