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Tina:Osteogenic differentiation on mES brainbow cells (OSG)
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=='''Seeding density test (OSG_E1)'''== *'''Purpose:''' To determine the seeding density of mESCs for osteogenic differentiation <br> === Test #1 (OSG_E1_T1) === *Date: 7/5/2012 ~ 7/7/2012 *Cells: P11 InsCAGGsAlov3 (mES brainbow cells) *7/5: P10 mES brainbow cells (on feeder) were trypsinized. Cells were then divided into two groups. Group one were seeded 1:2, 1:4, 1:6, 1:8 and 1:12 on gelatin coated glass-well immediately. Group two were let sit for 15 min and then seeded 1:2, 1:4, and 1:6 on gelatin coated glass-well. *7/6 (next day of seeding): Take pictures of each well. Find out the best seeding manner and seeding density. *According to results, seeding at 1:6 with 15 min sitting time is the best seeding density for osteogenic induction (one day after seeding).
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