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==Conclusion== *Amplification is successful, yet page quantification reviews that the band occurs around 150bp, which is not that right size of the library. According to expicentre, the correct size of the library should be ~175-500bp. Previous experiment with 100ng starting amount of gDNA show bands around 200 to 400bp. I think the band is most likely primer dimers. However when you view carefully at the gel image you will see a light smear of dna, that could be the library. Now if we can further enrich the yield of the library, we can possibly get a good amplicon out of it. I contacted Zymo's tech suport and Zeith was able to help me trouble shoot the problem, and he suggested a few improvements that can be made and could theoretically increase the yield. *Make new wash buffer *The washing step after binding can be omitted *Perform desulphonation step no longer than 15mins *warm the elution buffer up to 37C before use *Always use new CT conversion reagent
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