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===2nd Strand Synthesis and Library Enrichment=== *Mix on ice 0.5 uL 0.5uM Short Orange 0.5 uL 0.5uM Short Blue 10 uL DNA 1 uL Klenow 25 uL KAPA Supermix 13 uL Water 50ul total *Incubate at 30C for 10 min *Add 1ul 10uM Orange Primer 1ul 10uM Blue Primer **Perform Manufacturer protocol for KAPA 95C 3m 95C 10s 58C 30s 72C 3m plate read Goto step 2 repeat 34 72C 10m Hold 15C **Cycle the samples in a thermocycler under the following conditions *3. Purify the tagged DNA using qiaquick column *Page Quantification [[File:ZhangLab_2 2011-05-20 11hr 32min.jpg|300px]]
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