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=== August 24 === ==== qMDA BSA test ==== Motivation: We still need to solve the problem of missing DNA after extraction from the microwell arrays. * If an array is loaded with 10 ng/ul DNA, each 20 nl well should contain 200 pg. We are seeing much less than that by qMDA. * Many possibilities have been ruled out. ** Not due to shearing (8/22). ** Not due to DNA binding, time dependent (8/23). ** Not due to oil removal (8/23). * We now hypothesize that the BSA coating is the problem. Objective: Test whether BSA coating time affects PDMS microwell array retention of DNA. Secondary: Test whether a small amount of detergent improves recovery. Note: We have also gone back to using 10 ul of 1% BSA; Larger volumes are easier to spread over the array. We had been using 5 ul for several weeks. * 4 conditions: ** No BSA ** 30 min BSA ** 60 min BSA ** 60 min BSA + Tween *** DNA loaded also contained Tween [[File: 2013_08_26 BSA test qmda.xls| 2013_08_27 qmda BSA time and tween]] Conclusion: 30 min BSA condition showed less recovery than 60 min. No BSA showed even less. No significant difference between 60 min BSA with or without Tween.
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