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====Initial Imaging==== *Background signal did not play a factor since none overlapped with detected features during dcProbe0-Cy3 and dcProbe-FAM **We can say every feature we see is specific to 2nd rolony *dcProbe0-Cy3 and dcProbe0-FAM showed essentially no overlap, which gives us confidence these are true signals **Next time, we should be able to detect dcProbe1, dcProbe2, etc. and expect to see a fraction overlap with these *dcProbe0-Cy3 had 265 rolonies (~2.4/cell) *dcProbe0-FAM had 364 rolonies (~3.3/cell) *dcProbe0-Cy3(3641 probes) + dcProbe0-FAM (4097 probes) = 7,738 probes/12,964 total probes in Agi26k0gap = 60% **If these two dyes which can detect 60% of probes detected 629 2nd rolonies then we can approximate the total number of 2nd rolonies in this field of view = ~1050 *There are ~9.5 2nd rolonies/cell
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