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===PAGE Size Selection=== *Also include probes from [[Matt:LabNotes/2016-6-7]] [[File:2016-07-28_TB12kApr2016_V4_ProbeProduction_GelCheck.jpg|350px]] *You can see the 3 bands at the top *The full digested band is the brightest (third from top) and also kind of blurry due to amount *Run 6 gels for 6/7/2016 batch {| {{table}} | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''6X Volume''' |- | V4 Probes||95 |- | TBE-Urea Buffer 2X||95 |} *Run 9 gels for this batch (wanted to do 10 but not enough gels) {| {{table}} | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''9X Volume''' |- | V4 Probes||135 |- | TBE-Urea Buffer 2X||135 |} {| {{table}} | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''15X Volume''' |- | Low Mass Ladder||15 |- | TBE-Urea Buffer 2X||75 |- | H2O||60 |- | Total||150 |} *200V for 40min *No gel images because too many gels **Bands looked good, could see '''very''' faint band of ~200bp and a less faint band below that and finally the completely digested probe band was brightest below that
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