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==Discussion== *All libraires were working as we expected. *For the C5 sample, '''it is unknown why 10th cycle amplification has less background than 8th cycle amplification'''. I run another gel to check them (results not shown) and results are still the same. *The libraries C4 and C5 (from single cell genome 11-1 and 12-1, respectively) were ready for sequencing. **16S-PCR info: http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-1-28 **After discussed with Dr. Zhang, we will prefer to sequence sample from E.coli single cell genome in which we can optimized the protocol of library construction. **We hold the sequencing of the C4 and C5 librery.
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