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Tina:Osteogenic differentiation on mES brainbow cells (OSG)
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=== Test #2 (OSG_E3_T2)=== *Date: 8/3/2012 ~ ongoing work *Cells: P13 InsCAGGsAlov3 (mES brainbow cells) *Osteogenic differentiation media: 5% serum in osteogenic differentiation media. *8/3: P12 mES brainbow cells (feeder free) were trpsinized (TrpLE), let sit for 15 min, and then seeded 1:18 onto gelatin coated 25T (now become P13). *8/4 (Day 0): PBS wash twice and then add induction media. *Change media daily (PBS wash once). *8/7 (Day 3): Collect sample from one 25T. Store at -80C.
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