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===Results=== *The bisulfite conversion pcr test failed for some reason. Mostly due to old sodium bisulfite reagent. It was stored at -20C as recommended, but it is better to work with freshly prepared bisulfite conversion reagents. Will spend more time to investigate on this if possible. *The 1st amplification worked out really nice, with the exact amplicon size as expected and negative control didn't get amplified. *However after the 2nd amplification, unspecific bands showed up for both the sample and the control. I suspect this is due to lost of samples due to purification using the column. *I would like to try Rui's protocol where she did purification with beads and she had pretty good results from it.
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