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===March 11=== * Vipul has ordered a new primer for the CEL-Seq project, identical to barcode #39 from Hashimshony et al. This primer has the 5' adapter and T7 promoter to begin with. ** It is actually 3 primers that I will use together in order to get the 3' terminal V (A, C, G) on the poly dT stretch. * I will use this primer along with our own (using Bst to incorporate the T7 promoter). * Vipul needs approximately 15 million reads per library, out of a 40 million theoretical maximum per lane on the GAIIx. I need to make sure that Vipul is aware that CEL-seq requires paired end sequencing, which means that he may have to wait a while to get in on a run. * Vipul also want to do an Ampure XP bead purification on cDNA before IVT, rather than the cDNA column included with the kit. This does not make sense to me, since he is comparing his own method to CEL-Seq, and therefore improving or otherwise modifying the CEL-Seq protocol would not give any advantage in answering his reviewers comments.
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