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===Results=== *All imaging was done with 5% Laser and Gain of 550 **C10_100pM had a Z-thickness of ~37 microns so tried taking both 20 and 30 z-slices ***As seen in the table it didn't make a big difference *The disparity between C10 and D10 (both 100pM) can be partly attributed to different number of cells in field of view {| {{table}} | align="center" style="background:#f0f0f0;"|'''C10''' | align="center" style="background:#f0f0f0;"|'''D10''' |- | [[File:C10_100pM_G550_Cy3_z12_ch01.png|450px]]||[[File:D10_100pM_G550_30slices_Cy3_z09_ch01.png|450px]] |} {| {{table}} | align="center" style="background:#f0f0f0;"|'''Image''' | align="center" style="background:#f0f0f0;"|'''Rolony Count''' |- | C8_NegCtrl_G550_Cy3_ch00_MIP||0 |- | C9_NegCtrl_G550_Cy3_ch00_MIP||0 |- | D8_10pM_G550_Cy3_ch00_MIP||51 |- | D9_10pM_G550_Cy3_ch00_MIP||101 |- | E8_50pM_G550_Cy3_ch00_MIP||355 |- | E9_50pM_G550_Cy3_ch00_MIP||764 |- | C10_100pM_G550_Cy3_ch00_MIP||3216 |- | D10_100pM_G550_30slices_Cy3_ch00_MIP||521 |- | D10_100pM_G550_Cy3_ch00_MIP||497 |- | E10_10nM_G550_Cy3_ch00_MIP||17509 |- | F9_10nM_G550_Cy3_ch00_MIP||27746 |} [[File:PpConcTest_03242014.JPG]]
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