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== Bisulfite conversion == * Bisulfite conversion kit: EZ DNA Methylation-Lightning Kit (column-based method) , cat# D5030 or D5031 * EZ-96 DNA Methylation-Lightning™ MagPrep (magnetic bead-based method) is another option for large sample set * I followed the instruction of bisulfite conversion exact the same as describe in the manual. * Few things to note: :- Incubate with desulfonation buffer for 18min. :- Discard spnt after binding step and first washing. :- Spin for 2min for second washing. :- Elute with 33ul M-Elution Buffer. The total volume left should be ~31-32ul :- Transfer 30ul of bisulfite converted DNA to strip tube to be easy to handle during PCR step
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