Editing
Tina:Brainbow induction with Cre recombinase (BBI)
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
=== Test #1 (BBI_E4_T1) === *Date: 4/10/2013 ~ 4/12/2013 *Cells: P10 H9Bb3. H9 ESC brainbow clone #3. Feeder free culture system. Cell seeded on 4/10 in three glass bottom wells. *Results: experiment terminated. Cell type is not suitable for adenovirus infection. Because adenovirus will not integrate into host genome, proliferating cells (ESCs here) are not good starting material for Ad-Cre infection. Most of the cells won't be expressing Cre after 2-3 days. Will try to use differentiated cells (ESC-derived osteoblasts) next time. As long as cells are not proliferating, most of the cells should be able to express Cre.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information