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==anneal with AluI oligo and digestion with AluI 2008-08-19== 1. Add 1ul NEB buffer 1.5, 5 uL of 100 uM RE-AluI-V6_2_Ida guide oligo in 5ul circularized probe from previous steps(ratio is :1). 2. Denature at 95C in PCR machine for 10 mins. Turn off the machine and wait for 20 min. 2. Add 3.5uL of AluI restriction enzyme to reaction, incubate at 37C for about 2 hours. [[Image:20080820_digestion with AluI_oligo_.jpg]]20080820_digestion with AluI_oligo
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