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====Notes==== *Laser intensity = 1%, Cy3 gain = 600 (tried increasing gain but only increased background) *Z-stack system optimized for 63X objective (0.30um step size) *Imaged Samples 1, 2, and 3 in that order *Imaged each from bottom of white matter to top-left of white matter *Sample 1 also imaged up-left of white matter to show degradation and lack of rolonies in grey matter *Sample 1 had thickness <10um, thicker in white matter obviously *Sample 2 shows odd round objects in BF image when in white matter area **For some reason these are not seen in areas that are definite grey matter but I am not sure where the white/grey matter boundary is *Sample 3 had thickest z-stack probably because gel was made thicker and rolonies were generated in gel but above tissue
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