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== Screen more clones for Sanger sequencing == - Perform size screen of the PCR products from 2012_06_05 in 2% agarose gel, load sample 4ul each well (24 clones from 1U) [[File:2012_06_06_Size-screening_1U-1-12.png| 400px]] [[File:2012_06_06_Size-screening_1U-12-24.png| 400px]] - Purified 30ul of PCR products with 1vol. AmPure beads and eluted with 30ul EB buffer - Measured DNA conc. by Nanodrop - I send more 18 clones of 1U (1U-11 - 1U-28) for Sanger sequencing '''Tracking number: 10-196614018 (Tagging_Validation_2012_06_06)''', under Dinh's account '''PO#: 90443171:''' generated by Rui (Total 60, 2012_06_04 send 10 samples, 2012_06_05 send 32 samples , 2012_06_06 send 18 samples => 0 reactions left) * Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-6-7
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