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===Analysis description and links=== We collected 1064 single neuronal nucleus isolated from human brain Brodmann area 8. After a quality filter based on the difference between zero- and single-nucleus samples, 986 single nuclei were qualified to continue with clustering analysis. To identify neuornal cell types among these 986 samples, we measured similarity among expression profiles of all individual nuclei. The heat map reflecting correlation of each individual to all the others were used to guide for clustering. Correlations revealed two major nuclei population, which was differed significantly in genes expressed in GABAergic inhibitory neurons (GAD1/GAD2 etc.). The separation of these two clusters were further confirmed by K-means clustering and principle component analysis (PCA). We therefore performed a series of unbiased clustering approach that allows hierarchical bi-branching of samples based on gene expression profiles. By doing this, local rather than global gene expression pattern can be associated and subsets of nuclei can be identified based on genes with similar expression. All together, we divided 986 single-nucleus samples into 12 groups and identified 193 signature genes highly expressed in each defined group. [[File:12groups.jpg|500px]][[File:400genes_comp.jpg|500px]]
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